منابع مشابه
Studies on Lysosomes
Subcellular fractions were isolated by differential centrifugation from pure suspensions of human blood lymphocytes incubated with and without phytohemagglutinin (PHA). Between 30 and 120 min after addition of PHA to intact cells, redistribution of acid hydrolases (beta glucuronidase, acid phosphatase), from a 20,000 g x 20 min granular fraction into the corresponding supernatant, was observed....
متن کاملStudies on the Release of Lysosomal Enzymes from Kidney Lysosomes
Incubation of kidney lysosomes at 37 degrees results in a graded release of lysosomal enzymes. The release of enzyme occurs in two stages. First the enzymes become available to the substrate but remain sedimentable. Later the amount of soluble enzyme increases and eventually is almost equal to that of the available enzyme. Morphological studies of lysosomes showed that during the process involv...
متن کاملTurnover studies on proteins of rat liver lysosomes.
The turnover of rat liver lysosomal proteins was studied by a double isotope-labeling technique. The cellular fractions investigated included soluble lysosomal proteins, lysosomal membrane proteins, highly purified lysosomal beta-glucuronidase, and for comparison, microsomal proteins and soluble cytoplasmic proteins. Both "normal" lysosomes and Triton WR-1339-filled lysosomes (tritosomes) were ...
متن کاملSTUDIES ON LYSOSOMES V. The Effects of Streptolysins and Other Hemolytic Agents on Isolated Leucocyte Granules
Granules from rabbit peritoneal leucocytes were prepared in 0.3 ~ sucrose as an optically homogeneous suspension with the aid of heparin. Lysis of the granules in vitro was followed by measurement of decreases in the apparent absorbance of the suspensions at 520 m# and was accompanied by solubilization of beta-glucuronidase from the particles. Streptolysins O and S from hemolytic streptococci l...
متن کاملSTUDIES ON LYSOSOMES V. The Effects of Streptolysins and Other Hemolytic Agents on Isolated Leucocyte Granules
Granules from rabbit peritoneal leucocytes were prepared in 0.3 ~ sucrose as an optically homogeneous suspension with the aid of heparin. Lysis of the granules in vitro was followed by measurement of decreases in the apparent absorbance of the suspensions at 520 m# and was accompanied by solubilization of beta-glucuronidase from the particles. Streptolysins O and S from hemolytic streptococci l...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1962
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.116.4.433